Griseolic acids B and C, an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase.
نویسندگان
چکیده
griseoaurantiacus SANK63479. In this paper, we report the isolation procedure and structural elucidation of griseolic acids B2) and C3). Griseolic acids B and C were separated from the mother liquid of griseolic acid A by Sephadex G-10 column chromatography. The mother liquid was dissolved in water and applied on Sephadex G-10 column and developed with water. Two active fractions (griseolic acids B and C) were individually chromatographed on a column of DEAE-Sephadex A-25. After
منابع مشابه
Griseolic acid, an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase. II. The structure of griseolic acid.
Griseolic acid, a potent inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase, was isolated from the fermentation broth of Streptomyces griseoaurantiacus SANK 63479. Treatment of griseolic acid with HCl-MeOH gave adenine and pseudo-sugar. The structure of griseolic acid, adenine nucleoside type structure, was elucidated by chemical degradation and X-ray analysis, and was shown to...
متن کاملStudies on griseolic acid derivatives. VII. Synthesis and phosphodiesterase inhibitory activity of the C4'-C5' hydrogenated products of griseolic acid and their base-exchanged derivatives.
Addition reactions of the C4'-C5' double bond of griseolic acid were investigated. C4'-C5' Dihydrogriseolic acid was obtained by reduction of the adduct having halogen at the 4'-position. The ring juncture of the two five-membered rings of the C4'-C5' dihydro derivatives was of all-"cis" configuration. Acetolysis of the protected dihydrogriseolic acid gave the corresponding 1 '-acetoxy sugar de...
متن کاملStudies on griseolic acid derivatives. V. Synthesis and phosphodiesterase inhibitory activity of substituted derivatives of the hydroxy group at the 2'- or 7'-position in griseolic acid.
Substituted derivatives of the hydroxy group at the 2'or 7'-position in griseolic acid (1) were synthesized by selective substitution in order to study the relationship between structure and inhibitory activity against adenosine 3',5'-cyclic monophosphate (cAMP) or guanosine 3',5 '-cyclic monophosphate (cGMP) phosphodiesterase (PDE). All of the derivatives which had a configurationally inverted...
متن کاملExpression of Recombinant Phosphodiesterase 3A and 3B Using Baculovirus Expression System
Background: Phosphodiesterase 3A (PDE3A) and phosphodiesterase 3B (PDE3B) play a critical role in the regulation of intracellular level of adenosine 3´,5´-cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3´,5´-cyclic monophosphate (cyclic GMP, cGMP). Subsequently PDE3 inhibitors have shown to relax vascular and inhibit platelet aggregation in cardiovascular disease. Objectives: In th...
متن کاملAdenosine-mediated elevation of cyclic 3':5'-adenosine monophosphate concentrations in cultured mouse neuroblastoma cells.
Adenosine causes an increase in the concentration of cyclic AMP in mouse neuroblastoma cells. The amount of increase observed in intracellular cyclic AMP levels due to exogenous adenosine depends greatly on the concentration of a specific cyclic AMP phosphodiesterase inhibitor, 4-(-3-butoxy-4-methoxybenzyl)-2-imidazolidinone. Unstimulated concentrations of cyclic AMP were 29-40 pmol/mg of prote...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of antibiotics
دوره 41 5 شماره
صفحات -
تاریخ انتشار 1988